Isolation and structural elucidation of compounds from the non-alkaloidal extract of Nicandra physaloides and the antimicrobial activity of withanicandrin

The aerial parts of Nicandra physaloides plant collected from Kenyatta National Hospital grounds were dried and subjected to acid-base extraction and partitioned to obtain alkaloidal and non-alkaloidal extracts. The  non-alkaloidal extract yielded three compounds; withanicandrin, â-sitosterol and stigmasterol after column chromatography. Withanicandrin exhibited antifungal activity against Saccharomyces cerevisiae and Candida albicans but lacked antibacterial activity.Key words: Nicandra physaloides, withanicandrin, β-sitosterol, stigmasterol, antifungal activity

Investigation of Possible Antidotal Effects of Activated Charcoal, Sodium Bicarbonate, Hydrogen Peroxide and Potassium Permanganate in Zinc Phosphide Poisoning

Zinc phosphide, a commonly used rat poison in Kenya was mixed with maize flour in a concentration of 0.15 % w/w and fed to a group of 60 experimental mice for 3 hours. The mice were then randomly divided into 5 equal groups, A, B, C, D and E. To group A, B, C and D was administered 1 ml of activated charcoal (3 % w/v), sodium bicarbonate (10 % w/v), hydrogen peroxide (0.5 % v/v) and potassium permanganate (1:5000), respectively. Group E was given 1 ml distilled water and used as control. All five groups were observed for symptoms of toxicity, often culminating in death. The observations were continued over a period of 36 hours. Results of the experiment showed that all 4 test substances minimized the lethal effect of zinc phosphide. Although no attempt was made to quantify the antidotal effect of the 4 substances, activated charcoal appeared to have a higher effect than the others, while potassium permanganate had a low rating. Key Words: Zinc phosphide, rat poison, antidote East and Central African Journal of Pharmaceutical Sciences Vol.5(2) 2002: 38-4

Drug Quality Control in Kenya: Observation in Drug Analysis and Research Unit during the Period 1996-2000

The Drug Analysis and Research Unit received and analyzed 261 drug samples over a five-year period, 1996 to 2000. Samples were received from regulatory authorities, local industry, non-governmental organizations, hospitals and private practitioners. The samples analyzed constituted 59.8 % locally manufactured and 40.2 % imported products. The overall rate of failure to comply with quality specifications set out in the respective monographs was 21.1 %. This represents 24.6 % and 16.2 % of the locally manufactured and imported drugs, respectively. Key Words: Quality control, active pharmaceutical ingredient content, dissolution. East and Central African Journal of Pharmaceutical Sciences Vol.5(2) 2002: 28-3

Quality of Antiretroviral Drugs Analyzed in the Drug Analysis and Research Unit During 2000-2003

During the period 2000-2003 the Drug Analysis and Research Unit received and analyzed 33 samples of antiretroviral drugs. Locally manufactured products accounted for 57.6 % of the samples, while the imported drugs constituted 42.4 %. The drugs consisted of single, double and triple component preparations. They were subjected to the identity, assay and dissolution tests. 30 samples (90.9 %) complied with compedial specifications for these tests, while 3 failed. The results obtained show that manufacture of quality generic antiretroviral drugs is achievable. Key Words: Antiretroviral, assay, dissolution, nucleoside reverse-transcriptase inhibitor East & Central African Journal of Pharmaceutical Sciences Vol.6(1) 2003: 20-2

Drug Quality Control in Kenya: Observation in the Drug Analysis and Research Unit During the Period 2001-2005

During the five-year period January 2001 to December 2005, the Drug Analysis and Research Unit received and analyzed 394 drug samples. Samples were received from regulatory authorities, local industry, non-governmental organizations, hospitals and private practitioners. The samples analyzed constituted 37.8 % locally manufactured and 62.2 % imported products. In contrast to previous years when failure rates of over 20 % were recorded, the overall rate of failure to comply with compendial quality specifications was 6.1 %, comprising of 8.7 % locally manufactured and 4.5 % imported drugs

Quality Performance of Drugs Analyzed in the Drug Analysis and Research Unit (DARU) during the Period 2006-2010

During the period 2006-2010, the Drug Analysis and Research Unit analyzed 583 samples. The samples comprised 50.6% local and 49.4% imported products. Samples were subjected to compendial or in-house specifications. The failure rate was 12.2% for local products and 14.2% for imports.  Antibacterial products recorded the highest failure rate (21.6%) while  anticancers and drugs acting on the gastrointestinal, respiratory and  reproductive systems all passed in the tests performed. The failure rate for antiprotozoals, antimalarials, antifungals, anthelminthics and analgesics was 14.3%, 12.5%, 11.8%, 8.9% and 11.5%, respectively.Key words: DARU, drug product, assay, dissolution, antimicrobial, antimalaria